8 steps
Step 1 - Take the toxin gene from a stretch of Bt DNA and combine it with a vectorThe DNA of the gene and the DNA of the vector now form a continuous loop of DNA
Step 2 - add the vector to the bacterium Agro-bacterium, a bacterium that causes disease in plants, has the ability to transfer the portion of its DNA into plant cells. Once inside a plant cell, it's DNA will be incorporated into DNA of one of the plant's chromosomes.
Step 3 - move the bacteria to the growth medium Soon there are a million or more copied of the bacterium and of the rector that contains the Bt gene.
Step 4 - add pieces of the tomato plant's leaf to the bacteria The DNA of the bacteria enters the cells of the leaf and the cells' nuclei. The vector's DNA (which includes the Bt and herbicide-resistant genes) becomes integrated with the plant cells' DNA
Step 5 - move the plant cells to the growth medium for plants This growth medium promotes the regeneration of plants. As the plant cells multiply, they form stems, roots, and new leaves
Step 6 - spray herbicide on the plant cuttings Only cells with the herbicide-resistant gene survive. The Bt gene is present only in the plant cells that include the herbicide-resistant gene are capable of growing
Step 7 - transfer the plant to the growth chamber The generAted cutting is planted in soul and allowed to mature.
Step 8 - examine the plant to determine if it has the desired trait To test if this tomato plant is resistant to a targeted pest, the pest was allowed to eat the plant's leaves. Since only plants that have successfully incorporated the Bt gene will kill the pest, the death of this and the other caterpillars indicates that your were successful
Step 2 - add the vector to the bacterium Agro-bacterium, a bacterium that causes disease in plants, has the ability to transfer the portion of its DNA into plant cells. Once inside a plant cell, it's DNA will be incorporated into DNA of one of the plant's chromosomes.
Step 3 - move the bacteria to the growth medium Soon there are a million or more copied of the bacterium and of the rector that contains the Bt gene.
Step 4 - add pieces of the tomato plant's leaf to the bacteria The DNA of the bacteria enters the cells of the leaf and the cells' nuclei. The vector's DNA (which includes the Bt and herbicide-resistant genes) becomes integrated with the plant cells' DNA
Step 5 - move the plant cells to the growth medium for plants This growth medium promotes the regeneration of plants. As the plant cells multiply, they form stems, roots, and new leaves
Step 6 - spray herbicide on the plant cuttings Only cells with the herbicide-resistant gene survive. The Bt gene is present only in the plant cells that include the herbicide-resistant gene are capable of growing
Step 7 - transfer the plant to the growth chamber The generAted cutting is planted in soul and allowed to mature.
Step 8 - examine the plant to determine if it has the desired trait To test if this tomato plant is resistant to a targeted pest, the pest was allowed to eat the plant's leaves. Since only plants that have successfully incorporated the Bt gene will kill the pest, the death of this and the other caterpillars indicates that your were successful